The complete chloroplast genome of the wild thistle Cynara humilis L. (Asteraceae) is presented in this study. The genome is 152.585 bp in length and has a quadripartite structure composed by a large single-copy (LSC) of 83.622 bp, a small single-copy (SSC) of 18.651 bp and two inverted repeats (IRb/a) of 25.156 bp each. The GC content corresponds to 37.7%. The amount of unique genes is 114, in which 17 are duplicated in the IRs, for a total of 131 genes. A maximum parsimony phylogenetic analysis revealed that C. humilis chloroplast genome is closely related to that of the globe artichoke within the Carduoideae subfamily
