Using Arabidopsis-related model species (ARMS): growth, genetic transformation, and comparative genomics
Batelli G, Oh Dh, Paino D’Urzo M, Orsini F, Dassanayake M, Zhu Jk, Bohnert HJ, Bressan RA, Maggio A
In: “Arabidopsis Protocols, Methods in Molecular Biology” (Sanchez-Serrano JJ, Salinas J eds), vol. 1062, pp. 27-51. (2014) doi: 10.1007/978-1-62703-580-4_2
The Arabidopsis-related model species (ARMS) Thellungiella salsuginea and Thellungiella parvula have generated broad interest in salt stress research. While general growth characteristics of these species are similar toArabidopsis, some aspects of their life cycle require particular attention in order to obtain healthy plants, with a large production of seeds in a relatively short time. This chapter describes basic procedures for growth, maintenance, andAgrobacterium-mediated transformation of ARMS. Where appropriate, differences in requirements betweenThellungiella spp. and Arabidopsis are highlighted, along with basic growth requirements of other less studied candidate model species. Current techniques for comparative genomics analysis between Arabidopsis and ARMS are also described in detail.
