National Research Council of Italy

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IBBR publication #785

Characterization of microsatellite markers in Fagus sylvatica L. and Fagus orientalis Lipsky

Pastorelli R, Smulders MJ, Van Westende WP, Vosman B, Giannini R, Vettori C, Vendramin GG

Molecular Ecology Notes 3 (1): 76-78. (2003)
doi: 10.1046/j.1471-8286.2003.00355.x

Using an enrichment procedure, we cloned microsatellite repeats from European beech (Fagus sylvatica L.) and developed primers for the amplification of microsatellite markers. Six polymorphic loci were characterized which produced 3–21 alleles in 70 individuals from one Italian population, with an observed heterozygosity between 0.58 and 0.85. All six loci amplified fragments which were polymorphic in the closely related species,Fagus orientalis, also. Owing to their very high degree of variation, these markers should be very useful in gene flow studies of these species

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