Gianna Palmieri

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• Education

1989 Degree in Chemistry achieved at the University of Naples “Federico II”, final mark: 110/110 cum laude.
1990 Approval to the profession of Chemist.
1992/94 3-year specialization degree in “Advanced Biotechnology

• Employment and experience

1987/1989 Post-degree stage at University of Naples “Federico II”, Department of Organic and Biological Chemistry (DCOB)
1989/91 Two-years National Council of Research (CNR) fellowship at the Institute ICMIB, Naples.
1990/91 Visiting scientist at Department of Chemistry, Massachusetts Institute of Technology (MIT), Boston (USA).
1991/92 Scientific collaboration contract by CEINGE Biotecnologie Avanzate S.C.a r.l., Naples.
1992/94 3-year specialization degree in “Advanced Biotechnology”
1995/96 One-year CNR fellowship at University of Naples, DCOB.
1996/97 Scientific contract at the University of Naples, DCOB.
1997/98 Senior University fellowship, at DCOB.
1998/99 Scientific Contract as senior researcher, at the University of Naples, DCOB in the Project of Science and Technology Park.
1999/00 Scientific Contract as senior researcher, at the University of Naples, DCOB.
2000 Research staff scientist, IABBAM-CNR Institute, Naples.
2005 Research staff scientist, IBBR-CNR Institute, Naples

• Teaching

Dr. Palmieri, has given several experimental lessons and educational seminars for students in Chemistry during the academic years from 1997 to 2003. She also organized several seminars at the School of “Specialization in biotechnology applications” and for students in Chemistry. Since 1991 she has assisted in the preparation of the thesis many students in biology and chemistry, PhD and specializing students. During the academic year 1999/2000 she was the teacher of the course ’Bioanalytical Chemistry’ degree in Biotechnology.

• Brief description of research activities

Dr. Gianna Palmieri has achieved her master degree in Chemistry at the University of Naples and she became researcher at the National Research Council (CNR) in 2000. Her scientific and research activity has been focused on the study of the structure/function relationship of proteins from different sources with the aim to obtain biotechnological tools for industrial applications within different fields. In particular, Dr Palmieri has studied for a long time enzymes from basidiomycetes fungi involved in lignin bio-degradation. This research activity has allowed acquiring a consolidated know-how concerning the protein purification procedures as well as kinetic, chemical-physical, and structural characterization of enzymes involving in degradation of biopolymers. In the last ten years she focused her attention on proteolytic systems and specifically on the Acyl Peptide Hydrolases (APEH) studying its involvement in proteasome down-regulation for the development of new anticancer strategies. In addition she functionally and structurally characterized the first APEHs from different Antarctic fishes evidencing a new important role of this class of enzymes as antioxidant defence enzymatic systems. All these activities produced about 50 publications on peer-reviewed journals and 80 contributions to conference proceedings, which have received more than 2000 citations. She is co-inventor of 2 International Patents (1. PCT/EP2001/009147, Waste water treatment method using microorganisms. Inventors: Sannia G., Giardina P., Palmieri G., Mountadar M., Assobehei O., Kissi M., Mele A. 2. PCT/IT2009/000478. Proteases targeting agents. Inventors: Rossi M., Ruvo M., Palmieri G.)
Dr. Palmieri has a long and strong scientific background, developed over 26 years of growing experience acquired by intense training in Italian and international laboratories of both academic and industry institutions. In particular she has attended the laboratory of Prof. A. M. Klibanov, at the Massachusetts Institute of Technology (MIT), Boston (USA), and she worked in industrial research laboratories, in the project of’ high quality training of scientist for advanced biotechnologies. She is reviewer of several international journals as Journal of Protein Research. PLOS one; Applied Environmental Microbiology; Free Radical Biology & Medicine; Microbiology Letters; Biochimica et Biophysica Acta; Extremophiles.

• Selected Peer-reviewed Publications

P. Giardina, R. Cannio, L. Martirani, L. Marzullo, G. Palmieri and G. Sannia. Cloning and sequencing of a laccase gene from the lignin-degrading basidiomycete Pleurotus ostreatus. Appl. Environ. Microbiol. (1995) 61, 2408-2413.

G. Palmieri, M. Di Palo, A. Scaloni, S. Orrù, G. Marino and G. Sannia. Glutamate 1-semiadehyde aminotransferase from Sulfolobus solfataricus. Biochem. J. (1996) 320, 541-545.

G. Palmieri, P. Giardina, C. Bianco, A. Scaloni, A. Capasso, e G. Sannia. A novel white laccase from Pleurotus ostreatus. J. Biol. Chem. (1997) 272, 31301-31307 .

P. Giardina, G. Palmieri, A. Scaloni, B. Fontanella, V. Faraco, G. Cennamo, and G. Sannia. Protein and gene structure of a blue laccase from Pleurotus ostreatus. Biochem. J. (1999) 341, 655-663.

G. Palmieri, P. Giardina, B. Fontanella, C. Bianco, and G. Sannia. Copper induction of laccase isoenzymes in the lignonolytic fungus Pleurotus ostreatus. Appl. Environ. Microbiol. (2000) 66, 920-924.

G. Palmieri, C. Bianco, G. Cennamo, P. Giardina, G. Marino, M. Monti and G. Sannia. Purification characterization and functional role of a novel extracellular protease from Pleurotus ostreatus. Appl. Environ. Microbiol. (2001) 67 (6), 2754-2759.

V. Faraco, G. Palmieri, G. Festa, M. Monti, G. Sannia and P. Giardina. A new subfamily of fungal subtilases: structural and functional analysis of a Pleurotus ostreatus member. Microbiology. (2005) 151 (2), 457-466

G. Palmieri, G. Catara, M. Saviano, E. Langella, M. Gogliettino, and M. Rossi. First Archaeal PEPB-Serine Protease Inhibitor from Sulfolobus solfataricus with Non-Canonical Amino Acid Sequence in the Reactive-Site Loop. J. Proteome Res. (2009) 8(1), 327-34.

G. Palmieri, R. Cannio, I. Fiume, M. Rossi, G. Pocsfalvi. Outside the unusual cell wall of hyperthermophilic archaeon Aeropyrum pernix. Mol. Cell. Proteomics. (2009) 8, 2570-2581.

M. Gogliettino, M. Balestrieri, G. Pocsfalvi, I. Fiume, L. Natale, M. Rossi and G. Palmieri. A highly selective oligopeptide binding protein from the archaeon Sulfolobus solfataricus. J. Bacteriol. (2010) 192, 3123-3131

M. Balestrieri, M. Gogliettino, I. Fiume, G. Pocsfalvi, G. Catara, M. Rossi, G. Palmieri. Structural and functional insights into OppAAp from Aeropyrum Pernix: A member of a novel Archaeal OppA-subfamily. J. Bacteriol. (2011) 193, 620-630.

P. Bergamo, M. Gogliettino, G. Palmieri, E. Cocca, F. Maurano, R. Stefanile,M. Balestrieri, G. Mazzarella, C. David, and M. Rossi. Conjugated Linoleic Acid protects against gliadin-induced depletion of intestinal defenses. Mol. Nutr. Food Res. (2011) 55, S248-S256.

G. Palmieri, P. Bergamo, M. Gogliettino, M. Ruvo, A. Sandomenico, E. Langella, A. Luini, R. Hedge, M. Saviano, M. Rossi. Acyl peptide hydrolase inhibition as targeted strategy to induce proteasomal dysfunction. PLoS One (2011) 6, e25888.

Sandomenico A, Russo A, Palmieri G, Bergamo P, Gogliettino M, Falcigno L, Ruvo M. Small peptide inhibitors of Acetyl-Peptide Hydrolase having an uncommon mechanism of inhibition and a stable bent conformation. J Med Chem. (2012) 55(5), 2102-2011. doi: 10.1021/jm2013375.

Gogliettino M, Balestrieri M, Cocca E, Mucerino S, Rossi M, Petrillo M, Mazzella E, Palmieri G. Identification and characterisation of a novel acylpeptide hydrolase from Sulfolobus solfataricus: structural and functional insights. PLoS One. (2012) (5), e37921.

G. Palmieri, M. Balestrieri, J. Peter-Katalinić, G. Pohlentz, M. Rossi, I. Fiume, G. Pocsfalvi Surface-exposed glycoproteins of hyperthermophilic Sulfolobus solfataricus P2 show a common N- glycosylation profile. J. Proteome Res. (2013), 12 (6), 2779-2790.

P.Bergamo, E. Cocca, R. Palumbo, M. Gogliettino, M. Rossi, G. Palmieri. RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10,c12. Conjugated Linoleic Acid isomer on A375 melanoma cells. PLoS One (2013) 8(11), e80900.

M. Gogliettino, A. Riccio, M. Balestrieri, E. Cocca, A. Facchiano, T.M. D’Arco, C. Tesoro, M. Rossi, G. Palmieri. A novel class of bifunctional Acylpeptide Hydrolases: potential role in the antioxidant defense systems of the Antarctic fish Trematomus bernacchii. FEBS J. (2014) 281, 401-415.

G. Mazzarella, P. Bergamo, F. Maurano, D. Luongo, V. R. Aufiero, G. Bozzella, G. Palmieri, R. Troncone, S. Auricchio, C. David and M. Rossi. Gliadin intake alters the small intestinal mucosa in indomethacin-treated HLA-DQ8 transgenic mice. AJP Gastrointestinal and Liver Physiology. (2014) 307, G9 -G11.

A. Sandomenico, V. Celentano, L.D. D’Andrea, G. Palmieri, M. Ruvo. Screening of β-hairpin peptide-engrafted 1.2,3- triazoles to identify APEH enzyme inhibitors. RSC Advances. (2015) RSC Adv. 5, 9965-9972

P. Bergamo, G. Palmieri, E. Cocca, I. Ferrandino, M. Gogliettino, A. Monaco, F. Maurano, and M. Rossi. Adaptive response activated by dietary cis9, trans11 Conjugated Linoleic Acid prevents distinct signs of gliadin-induced enteropathy in mice. Eur. J. Nutr. (2015) DOI 10.1007/s00394-015-0893-2

A. Riccio, M. Gogliettino, G. Palmieri, M. Balestrieri, A. Facchiano, M. Rossi, S. Palumbo, G. Monti and E. Cocca. A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. PLoS One (2015) 10(5), e0125594 DOI:10.1371/journal.pone.0125594

Selected Publications

(full list available at CNR People)

An Active Peptide-Based Packaging System to Improve the Freshness and Safety of Fish Products: A Case Study

Ambrosio RL, Gogliettino M, Agrillo B, Proroga YT, Balestrieri M, Gratino L, Cristiano D, Palmieri G, Anastasio A

Year: 2022

The Antimicrobial Peptide 1018-K6 Interacts Distinctly with Eukaryotic and Bacterial Membranes, the Basis of Its Specificity and Bactericidal Activity

Ambrosio RL, Rosselló CA, Casares D, Palmieri G, Anastasio A, Escribá PV

Year: 2022

Differential Distribution and Activity Profile of Acylpeptide Hydrolase in the Rat Seminiferous Epithelium

Covarrubias AA, De la Fuente-Ortega E, Rossi G, Cocca E, Rossi M, Palmieri G, Pancetti FC

Year: 2022

Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry

Gogliettino M, Arciello S, Cillo F, Carluccio AV, Palmieri G, Apone F, Ambrosio RL, Anastasio A, Gratino L, Carola A, Cocca E

Year: 2022

Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants

Molisso D, Coppola M, Buonanno M, Di Lelio I, Aprile AM, Langella E, Rigano MM, Francesca S, Chiaiese P, Palmieri G, Tatè R, Sinno M, Barra E, Becchimanzi A, Monti SM, Pennacchio F, Rao R

Year: 2022

Oxidized Substrates of APEH as a Tool to Study the Endoprotease Activity of the Enzyme

Sandomenico A, Gogliettino M, Iaccarino E, Fusco C, Caporale A, Ruvo M, Palmieri G, Cocca E

Year: 2022

In-silico evaluation of adenoviral COVID-19 vaccination protocols: Assessment of immunological memory up to 6 months after the third dose

Stolfi P, Castiglione F, Mastrostefano E, Di Biase I, Di Biase S, Palmieri G, Prisco A

Year: 2022

Key Physicochemical Determinants in the Antimicrobial Peptide RiLK1 Promote Amphipathic Structures

Falcigno L, D’auria G, Palmieri G, Gogliettino M, Agrillo B, Tatè R, Dardano P, Nicolais L, Balestrieri M

Year: 2021

A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica

Festa R, Ambrosio RL, Lamas A, Gratino L, Palmieri G, Franco CM, Cepeda A, Anastasio A

Year: 2021

A safe and multitasking antimicrobial decapeptide: the road from de novo design to structural and functional characterization

Agrillo B, Proroga YT, Gogliettino M, Balestrieri M, Tatè R, Nicolais L, Palmieri G

Year: 2020

The bactericidal activity of protein extracts from Loranthus europaeus Berries: a natural resource of bioactive compounds

Ambrosio RL, Gratino L, Mirino S, Cocca E, Pollio A, Anastasio A, Palmieri G, Balestrieri M, Genovese A, Gogliettino M

Year: 2020

Rapid biofilm eradication of the antimicrobial peptide 1018-K6 against Staphylococcus aureus: a new potential tool to fight bacterial biofilms

Colagiorgi A, Festa R, Di Ciccio PA, Gogliettino M, Balestrieri M, Palmieri G, Anastasio A, Ianieri A

Year: 2020

Extending the shelf-life of meat and dairy products via PET-modified packaging activated with the antimicrobial peptide MTP1

Gogliettino M, Balestrieri M, Ambrosio RL, Anastasio A, Smaldone G, Proroga YT, Moretta R, Rea I, De Stefano L, Agrillo B, Palmieri G

Year: 2020

New antimicrobial devices to prevent waterborne diseases

Palmieri G, Cammarano A, Agrillo B, Proroga YT, Gogliettino M, Balestrieri M, De Stefano L, Battisti M, De Martino S, Mercurio ME, Cummaro A, Nicolais L

Year: 2020

Functionalized polymeric materials with bio-derived antimicrobial peptides for “active” packaging

Agrillo B, Balestrieri M, Gogliettino M, Palmieri G, Moretta R, Proroga Y, Rea I, Cornacchia A, Capuano F, Smaldone G, De Stefano L

Year: 2019

An alternative biocontrol agent of soil-borne phytopathogens: A new antifungal compound produced by a plant growth promoting bacterium isolated from North Algeria

Agrillo B, Mirino S, Tatè R, Gratino L, Gogliettino M, Cocca E, Tabli N, Nabti E, Palmieri G

Year: 2019

Newer Technologies, Better Healthcare

De Stefano L, Rea I, Dardano P, Balestrieri M, Palmieri G, Agrillo B, Terracciano M, Battisti M, Cammarano A, Nicolais L

Year: 2019

The extraordinary resistance to UV radiations of a manganese superoxide dismutase of Deinococcus radiodurans offers promising potentialities in skin care applications

Palmieri G, Arciello S, Bimonte M, Carola A, Tito A, Gogliettino M, Cocca E, Fusco C, Balestrieri M, Colucci MG, Apone F

Year: 2019

Bactericidal and antibiofilm activity of bactenecin-derivative peptides against the food-pathogen Listeria monocytogenes : New perspectives for food processing industry

Palmieri G, Balestrieri M, Capuano F, Proroga YT, Pomilio F, Centorame P, Riccio A, Marrone R, Anastasio A

Year: 2018

Small synthetic peptides bioconjugated to hybrid gold nanoparticles destroy potentially deadly bacteria at submicromolar concentrations

Palmieri G, Tatè R, Gogliettino M, Balestrieri M, Rea I, Terracciano M, Proroga YT, Capuano F, Anastasio A, De Stefano L

Year: 2018

Unusual Antioxidant Properties of 26S Proteasome Isolated from Cold-Adapted Organisms

Gogliettino M, Cocca E, Fusco C, Agrillo B, Riccio A, Balestrieri M, Facchiano A, Pepe A, Palmieri G

Year: 2017

Isolation and characterization from solar salterns of North Algeria of a haloarchaeon producing a new halocin

Mazguene S, Rossi M, Gogliettino M, Palmieri G, Cocca E, Mirino S, Imadalou-Idres N, Benallaoua S

Year: 2017

Low Erythrocyte Levels of Proteasome and Acyl-Peptide Hydrolase (APEH) Activities in Alzheimer’s Disease: A Sign of Defective Proteostasis?

Palmieri G, Cocca E, Gogliettino M, Valentino R, Ruvo M, Cristofano G, Angiolillo A, Balestrieri M, Rossi M, Di Costanzo A

Year: 2017

Plant growth promoting and inducible antifungal activities of irrigation well water-bacteria

Tabli N, Rai A, Bensidhoum L, Palmieri G, Gogliettino M, Cocca E, Consiglio C, Cillo F, Bubici G, Nabti E

Year: 2017

Adaptive response activated by dietary cis9, trans11 conjugated linoleic acid prevents distinct signs of gliadin-induced enteropathy in mice

Bergamo P, Palmieri G, Cocca E, Ferrandino I, Gogliettino M, Monaco A, Maurano F, Rossi M

Year: 2016

NMR and computational data of two novel antimicrobial peptides

Falcigno L, Palmieri G, Balestrieri M, Proroga YT, Facchiano A, Riccio A, Capuano F, Marrone R, Campanile G, Anastasio A

Year: 2016

Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii

Gogliettino M, Balestrieri M, Riccio A, Facchiano A, Fusco C, Palazzo VC, Rossi M, Cocca E, Palmieri G

Year: 2016

New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence

Palmieri G, Balestrieri M, Proroga YT, Falcigno L, Facchiano A, Riccio A, Capuano F, Marrone R, Neglia G, Anastasio A

Year: 2016

APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome

Palumbo R, Gogliettino M, Cocca E, Iannitti R, Sandomenico A, Ruvo M, Balestrieri M, Rossi M, Palmieri G

Year: 2016

A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus

Riccio A, Gogliettino M, Palmieri G, Balestrieri M, Facchiano A, Rossi M, Palumbo S, Monti G, Cocca E

Year: 2015

Structural characterization and antimicrobial activity of innovative compounds: a novel strategy for in silico peptides design

Riccio A, Palmieri G, Proroga Y, Balestrieri M, Falcigno L, Nocera FP, Anastasio A

Year: 2015

Screening of β-hairpin peptide-engrafted 1.2,3-triazoles to identify APEH enzyme inhibitors

Sandomenico A, Celentano V, D’Andrea LD, Palmieri G, Ruvo M

Year: 2015

A novel class of bifunctional acylpeptide hydrolases

Gogliettino M, Riccio A, Balestrieri M, Cocca E, Facchiano A, D’arco Tm, Tesoro C, Rossi M, Palmieri G

Year: 2014

A new member of thermopsin family from Sulfolobus solfataricus possibly involved in signal-transduction pathways

Gogliettino M, Riccio A, Cocca E, Cecere Palazzo V, Tesoro C, Balestrieri M, Palmieri G, Rossi M

Year: 2014

A new pepstatin-insensitive thermopsin-like protease overproduced in peptide-rich cultures of sulfolobus solfataricus

Gogliettino M, Riccio A, Cocca E, Rossi M, Palmieri G, Balestrieri M

Year: 2014

Gliadin intake alters the small intestinal mucosa in indomethacin-treated HLA-DQ8 transgenic mice

Mazzarella G, Bergamo P, Maurano F, Luongo D, Rotondi Aufiero V, Bozzella G, Palmieri G, Troncone R, Auricchio S, David C, Rossi M

Year: 2014

RedOx Status, Proteasome and APEH: Insights into Anticancer Mechanisms of t10,c12-Conjugated Linoleic Acid Isomer on A375 Melanoma Cells

Bergamo P, Cocca E, Palumbo R, Gogliettino M, Rossi M, Palmieri G

Year: 2013

Surface-exposed Glycoproteins of Hyperthermophilic Sulfolobus solfataricus P2 Show a Common N-Glycosylation Profile

Palmieri G, Balestrieri M, Peter-Katalinic J, Pohlentz G, Rossi M, Fiume I, Pocsfalvi G

Year: 2013

Structural and Functional Insights into Aeropyrum pernix OppA, a Member of a Novel Archaeal OppA Subfamily

Balestrieri M, Gogliettino M, Fiume I, Pocsfalvi G, Catara G, Rossi M, Palmieri G

Year: 2011

Conjugated linoleic acid protects against gliadin-induced depletion of intestinal defenses

Bergamo P, Gogliettino M, Palmieri G, Cocca E, Maurano F, Stefanile R, Balestrieri M, Mazzarella G, David C, Rossi M

Year: 2011

Protease mediated processing of a Cu-induced laccase in Pleurotus ostreatus: a natural approach to improve protein stability

Giardina P, Cennamo G, Greco C, Palmieri G, Sannia G

Year: 2002

Structural and kinetic characterization of native laccases from Pleurotas ostreatus, Rigidoporus lignosus, and Trametes trogii

Garzillo AM, Colao MC, Buonocore V, Oliva R, Falcigno L, Saviano M, Santoro AM, Zappala R, Bonomo RP, Bianco C, Giardina P, Palmieri G, Sannia, G

Year: 2001

Purification, characterization, and functional role of a novel extracellular protease from Pleurotus ostreatus

Palmieri G, Bianco C, Cennamo G, Giardina P, Marino G, Monti M, Sannia, G

Year: 2001

Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus

Palmieri G, Giardina P, Bianco C, Fontanella B, Sannia, G

Year: 2000

A novel white laccase from Pleurotus ostreatus

Palmieri G, Giardina P, Bianco C, Scaloni A, Capasso A, Sannia, G

Year: 1997