*

Institute of Biosciences and Bioresources

National Research Council of Italy

Giuseppe Perugino

Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132496
E-mail: giuseppe.perugino@ibbr.cnr.it


Name PERUGINO Giuseppe

Address Via Pietro Castellino 111 - 80131, Napoli

Tel. +39 081 6132496

Fax +39 081 6132634

e-mail giuseppe.perugino@ibbr.cnr.it

alternative email g.perugino@icloud.com

Education

  • 2003 PhD Biological Chemistry and Molecular Biology, University of Naples “Federico II”, Faculty of Biological Sciences.
  • 1997 Degree in Biological Sciences, University of Naples “Federico II”, Faculty of Biological Sciences.

Work Experience

  • 2014 - today staff researcher at the Institute of Biosciences and BioResources (IBBR), National Research Council of Italy.
  • 01/01/2008 - 31/12/2013 staff researcher at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/05/2003 - 31/12/2007 research fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/11/1998 - 31/10/2002 PhD student at the University of Naples “Federico II”, Department of Organic and Biological Chemistry.
  • 15/06/1998 - 31/10/1998 Post lauream fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.

Keywords

Hyperthermophilic Archaea - thermostable proteins and enzymes - DNA repair enzymes - genome stability - biotechnology - protein tags - Carbohydrate Active Enzymes - oligosaccharide biosynthesis.

Topics

The genome safeguard in thermophilic organisms

Since 2007, Dr. Perugino has been involved in the study and the analysis of proteins related to the safeguard of the genomic stability of the archaeon Sulfolobus solfataricus, in collaboration with Dr. Maria Ciaramella and Dr. Anna Valenti. In particular, he draws his attention to the study of:

  1. the reaction mechanism of the reverse gyrase (SsRG), a peculiar topoisomerase
    exclusively found in microrganisms that thrive at high temperature, and solely able to induce positive supercoiling to covalently closed molecules of DNA. The activity of this enzyme seems to be also important in vivo for the tuning of physiological activities involving other DNA topoisomerases and some DNA polymerases. Same studies were performed on the RG homolog from Pyrobaculum calidifontis, which displays a different action, respect to the S. solfataricus enzyme;
  2. the in vivo and in vitro characterization of a small thermophilic protein, the O6-alkylguanine-DNA-alkyl-transferase from S. solfataricus (SsOGT). This enzyme belongs to a class of transferases (namely AGT, OGT, MGMT; EC 2.1.1.63) involved in the direct repair of O6-alkylated guanines in the DNA, upon the treatment with endogenous and exo-genous alkylating agents. AGTs are evolutionary conserved and show a peculiar reaction mechanism: the alkyl group is irreversibly transferred from the guanine nucleobase to the catalitic cysteine, making the enzyme inactive and prone to degradation cellular pathway. SsOGT was heterologous expressed in Escherichia coli: thanks to the exceptional stability of the Sulfolobus homologue, it was possible the achievement of 3D structures of the wild type
    enzyme in the free form, in methylated DNA::protein complex, and in the methylated protein after reaction, by the collaboration with the prof. Menico Rizzi’s lab (University of Piemonte Orientale - Novara, ITALY). For the first time, evident conformational changes upor protein alkylation were identified, sha-ding new light on several aspects concerning the protein stability upon the DNA damage repair and the subsequent alkylation of the protein.
    The complete biochemical characterization was performed by an innovative assay introduced for the first time in our lab, which makes use of
    fluorescent inhibitor derivatives of this class of proteins.The same method was successfully applied to other (hyper)thermophilic AGTs (from Pyrococcus furiosus and Thermotoga neapolitana), as well as the human, the E. coli and the Mycobacterium tuberculosis homologues and their relative mutants. This innovative methodology was successfully applied as in vitro on purified proteins, as in vivo in several model microorganisms.

Modern biotechnologies by employing DNA active enzymes

  1. The peculiar reaction mechanism of AGTs and the exceptional stability of thermophilic members of this class of enzymes led to propose the latters as good candidates for their employement as protein-tag, in order to expand the potentiality of a well-established biotechnology (the SNAP-tag® technology). To date, our group is unique in the production and characterization of thermostable AGTs and their utilization in extreme reaction contitions, as in vitro as in vivo in extremophilic model microorganisms (Thermus thermophilus HB27 and Sulfolobus strains).
  2. The SNAP-tag® technology uses particular benzyl-guanine (BG) substrates for the covalent lab
    elling of desired chemical group to protein of interest. However, these commercially available products, or opportunely customised, is an disadvantage in terms of time and costs. Recently, we proposed a chemo-enzymatic approach, which combines the specificity of the enzymatic reaction of AGTs to the chemical fast "click" cycloaddition, by using an azide-based BG substrate to (potentially) an infinite number of commercial DBCO-based compounds.n This innovative methodolgy resulted very specific and efficient also in protein extracts, as for the labelling by fluorescent probes, as for the covalent immobilization on DBCO-derivatised bio-chip supports.
  3. The thermoSNAP-tag (an engineered version of SsOGT) was fused to a transmembra
    ne protein (INPN) for the expression and in vivo immobilization of enzymes of interest (EOI) without the need of anypurification. This new protein-tag, called ASLtag (Anchoring and Self Labelling protein-tag)allows as the expression of the fused EOI and its directly exposition to the solvent, as well as taking advantage by the presence of the thermoSNAPtag which, in turn, can be covalently conjugated to molecules which tune the activity of the EOI. Moreover, ASLtag revealed thermo-protective on EIOs against their denaturation.

Publications

  • MERLO R, CAPRIOGLIO D, CILLO M, VALENTI A, MATTOSSOVICH R, MORRONE C, MASSAROTTI A, ROSSI F, MIGGIANO R, LEONARDI A, MINASSI A, PERUGINO G (2020). The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 36, p. 85-97, ISSN: 1475-6366, doi: 10.1080/14756366.2020.1841182
  • VISONE V, SZABÓ I, PERUGINO G, HUDECZ F, BÁNÓCZI Z, VALENTI A (2020). Topoisomerases inhibition and DNA binding mode of daunomycin-oligoarginine conjugate. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 35, p. 1363-1371, ISSN: 1475-6366, doi: 10.1080/14756366.2020.1780226
  • MATTOSSOVICH R, MERLO R, FONTANA A, d’IPPOLITO G, TERNS MP, WATTS EA, VALENTI A, PERUGINO G (2020). A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures. EXTREMOPHILES, vol. 24, p. 81-91, ISSN: 1431-0651, doi: 10.1007/s00792-019-01134-3
  • DEL PRETE S, MERLO R, VALENTI A, MATTOSSOVICH R, ROSSI M, CARGINALE V, SUPURAN CT, PERUGINO G, CAPASSO C. (2019). Thermostability enhancement of the a-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling- protein-tag system (ASLtag). JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 946-954, ISSN: 1475-6366, doi: 10.1080/14756366.2019.1605991
  • LO GULLO G, MATTOSSOVICH R, PERUGINO G, LA TEANA A, LONDEI P, BENELLI D (2019). Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate. ARCHAEA, vol. 1, p. 1-10, ISSN: 1472-3654, doi: 10.1155/2019/9848253
  • MERLO R, DEL PRETE S, VALENTI A, MATTOSSOVICH R, CARGINALE V, SUPURAN CT, CAPASSO C, PERUGINO G (2019). An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 490-499, ISSN: 1475-6366, doi: 10.1080/14756366.2018.1559161
  • PERUGINO G, STRAZZULLI A, MAZZONE M, ROSSI M, MORACCI M (2019). Effects of Random Mutagenesis and In Vivo Selection on the Specificity and Stability of a Thermozyme. CATALYSTS, vol. 9, p. 440-454, ISSN: 2073-4344, doi: 10.3390/catal9050440
  • SERPE M, FORENZA C, ADAMO A, RUSSO N, PERUGINO G, CIARAMELLA M, VALENTI A (2019). the DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) Of Caenorhabditis Elegans Is Involved In Meiosis And early Development Under physiological Conditions. SCIENTIFIC REPORTS, vol. 9, p. 6889-6903, ISSN: 2045-2322, doi: 10.1038/s41598-019-43394-1
  • STRAZZULLI A, PERUGINO G, MAZZONE M, ROSSI M, WITHERS SG, MORACCI M (2019). Probing the role of an invariant active site His in family GH1 ß-glycosidases. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 973-980, ISSN: 1475-6366, doi: 10.1080/14756366.2019.1608198
  • BETTOTTI P, VISONE V, LUNELLI L, PERUGINO G, CIARAMELLA M, VALENTI A (2018). Structure and Properties of DNA Molecules Over The Full Range of Biologically Relevant Supercoiling States. SCIENTIFIC REPORTS, vol. 8, p. 1-14, ISSN: 2045-2322, doi: 10.1038/s41598-018-24499-5
  • ROSSI F, MORRONE C, MASSAROTTI A, FERRARIS DM, VALENTI A, PERUGINO G, MIGGIANO G (2018). Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 500, p. 698-703, ISSN: 0006-291X, doi: 10.1016/j.bbrc.2018.04.139
  • MIGGIANO R, VALENTI A, FRANCA R, RIZZI M, PERUGINO G, CIARAMELLA M. (2017). Every OGT Is Illuminated ... by Fluorescent and Synchrotron Lights. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 18, p. 1-18, ISSN: 1422-0067, doi: 10.3390/ijms18122613
  • MORRONE C, MIGGIANO R, SERPE M, MASSAROTTI A., VALENTI A, DEL MONACO G, ROSSI M, ROSSI F, RIZZI M, PERUGINO G, CIARAMELLA M (2017). Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, vol. 1861, p. 86-96, ISSN: 0304-4165, doi: 10.1016/j.bbagen.2016.10.020
  • VISONE V, HAN W, PERUGINO G, DEL MONACO G, SHE Q, ROSSI M, VALENTI A, CIARAMELLA M.¿ (2017). In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag. PLOS ONE, vol. 12, p. 1-19, ISSN: 1932-6203, doi: journal.pone.0185791
  • MIGGIANO R, PERUGINO G, CIARAMELLA M, SERPE M, REJMAN D, PAV O, POHL R, GARAVAGLIA S, LAHIRI S, RIZZI M, and ROSSI F (2016). Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled onto damaged DNA. BIOCHEMICAL JOURNAL, vol. 473, p. 123-133, ISSN: 0264-6021, doi: 10.1042/BJ20150833
  • VETTONE A, SERPE M, HIDALGO A, BERENGUER J, DEL MONACO G, VALENTI A, ROSSI M, CIARAMELLA M, PERUGINO G (2016). A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA-alkyl- transferase by protein engineering. EXTREMOPHILES, vol. 20, p. 1-13, ISSN: 1431-0651, doi: 10.1007/s00792-015-0791-9
  • PERUGINO G, MIGGIANO R, SERPE M, VETTONE A, VALENTI A, LAHIRI S, ROSSI F, ROSSI M, RIZZI M, and CIARAMELLA M (2015). Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein. NUCLEIC ACIDS RESEARCH, vol. 43, p. 8801-8816, ISSN: 1362-4962, doi: 10.1093/nar/gkv774
  • JAMROZE A, PERUGINO G, VALENTI A, RASHID N, ROSSI M, AKHTAR M, and CIARAMELLA M (2014). The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, p. 3231-3243, ISSN: 0021-9258, doi: 10.1074/jbc.M113.517649
  • VETTONE A, PERUGINO G, ROSSI M, VALENTI A, and CIARAMELLA M (2014). Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. EXTREMOPHILES, vol. 18, p. 895-904, ISSN: 1431-0651, doi: 10.1007/s00792-014-0662-9
  • VISONE V, VETTONE A, SERPE M, VALENTI A, PERUGINO G, ROSSI M, and CIARAMELLA M (2014). Chromatin Structure and Dynamics in Hot Environments: Architectural Proteins and DNA Topoisomerases of Thermophilic Archaea. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 15, p. 17162-17187, ISSN: 1422-0067, doi: 10.3390/ijms150917162
  • MIGGIANO R, CASAZZA V, GARAVAGLIA S, CIARAMELLA M, PERUGINO G, RIZZI M, and ROSSI F. (2013). Biochemical and structural studies in the M. tubercolosis O6-methylguanine methyltransferase and mutated variants.. JOURNAL OF BACTERIOLOGY, vol. 195, p. 2728-2736, ISSN: 1098-5530, doi: 10.1128/JB.02298-12
  • COBUCCI-PONZANO B, PERUGINO G, STRAZZULLI A, ROSSI M, and MORACCI M (2012). Thermophilic Glycosynthases for Oligosaccharides Synthesis. METHODS IN ENZYMOLOGY, vol. 510, p. 273-300, ISSN: 0076-6879, doi: 10.1016/B978-0-12-415931-0.00015-X
  • PERUGINO G, VETTONE A, ILLIANO G, VALENTI A, FERRARA MC, ROSSI M, and CIARAMELLA M. (2012). Activity and Regulation of an Archaeal DNA-Alkyltransferase: a Conserved Protein Involved in Repair of DNA Alkylation Damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, p. 4222-4231, ISSN: 0021-9258
  • VALENTI A, DE FELICE M, PERUGINO G, BIZARD A, NADAL M, ROSSI M, and CIARAMELLA M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization.. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, p. 30282-30295, ISSN: 0021-9258, doi: 10.1074/jbc.M112.366377
  • COBUCCI-PONZANO B, PERUGINO G, ROSSI M, and MORACCI M (2011). Engineering the stability and the activity of a glycoside hydrolase. PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 24 (1-2), p. 21-26, ISSN: 1741-0126
  • VALENTI A, PERUGINO G, ROSSI M, and CIARAMELLA M (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 39, p. 58-63, ISSN: 0300-5127, doi: 10.1042/BST0390058
  • Cobucci Ponzano, Beatrice, PERUGINO, GIUSEPPE, Trincone, Antonio, Mazzone, Marialuisa, DI LAURO, BARBARA, Giordano, Assunta, ROSSI, MOSE’, MORACCI, Marco (2010). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1024-2422, doi: 10.1080/10242420310001618555
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, and ROSSI M (2010). Ischia (Naples) hosted the Eigth Carbohydrate Bioengineering Meeting on May 10-13, 2009. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 28, p. 156, ISSN: 1024-2422
  • PERUGINO G, MORACCI M, and ROSSI M (2010). THERMOPHILIC ENZYMES OF POTENTIAL INDUSTRIAL USE: STRUCTURE AND FUNCTION. In: PECHKOVA E. AND RIEKEL C.. SYNCHROTON RADIATION AND STRUCTURAL PROTEOMICS (Pan Stanford Series on Nanobiotechnology, Series Editor Prof. Nicolini C.). vol. 3, p. 319-355
  • PERUGINO G, VALENTI A, ROSSI M, and CIARAMELLA M (2010). NUOVE PROTEINE TERMOSTABILI PER LA MARCATURA SPECIFICA DI PROTEINE IN VIVO E IN VITRO. NA2010A000013, Consiglio Nazionale delle Ricerche
  • VALENTI A, PERUGINO G, VARRIALE A, D’AURIA S, ROSSI M, and CIARAMELLA M (2010). The Archaeal Topoisomerase Reverse Gyrase Is a Helix-destabilizing Protein That Unwinds Four-way DNA Junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, p. 36532-36541, ISSN: 0021-9258
  • PERUGINO G, VALENTI A, D’AMARO A, ROSSI M, and CIARAMELLA M (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 37, p. 69-73, ISSN: 0300-5127
  • VALENTI A, PERUGINO G, NOHMI T, ROSSI M, and CIARAMELLA M (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, vol. 37, p. 4287-4295, ISSN: 0305-1048, doi: 10.1093/nar/gkp386
  • DI LAURO B, STRAZZULLI A, PERUGINO G, LA CARA F, BEDINI E, CORSARO MM, ROSSI M, and MORACCI M (2008). Isolation and characterization of a new family 42 ß-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues. BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1784, p. 292-301, ISSN: 0006-3002, doi: 10.1016/j.bbapap.2007.10.013
  • VALENTI A, PERUGINO G, D’AMARO A, CACACE A, NAPOLI A, ROSSI M, and CIARAMELLA M (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, vol. 36, p. 4587-4597, ISSN: 0305-1048
  • AUSILI A, COBUCCI-PONZANO B, DI LAURO B, D’AVINO R, PERUGINO G, BERTOLI E, SCIRÈ A, ROSSI M, TANFANI F, and MORACCI M (2007). A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures. PROTEINS, vol. 67, p. 991-1001, ISSN: 0887-3585, doi: doi: 10.1002/prot.21368
  • Ausili, Alessio, Cobucci Ponzano, Beatrice, DI LAURO, BARBARA, D’Avino, Rossana, PERUGINO, GIUSEPPE, Bertoli, Enrico, Scirè, Andrea, ROSSI, MOSE’, Tanfani, Fabio, MORACCI, Marco (2007). A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures. PROTEINS, vol. 67, p. 991-1001-1001, ISSN: 0887-3585, doi: 10.1002/prot.21368
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, and ROSSI M (2007). Biotechnology. In: RICARDO CAVICCHIOLI. Archaea: molecular and cellular biology. p. 478-495, WASHINGTON, DC 20036-2904:America Society for Microbiology
  • GLOSTER TM, ROBERTS S, PERUGINO G, ROSSI M, MORACCI M, PANDAY N, TERINEK M, VASELLA A, and DAVIES GJ (2006). Structural, kinetic, and thermodynamic analysis of glucoimidazole-derived glycosidase inhibitors. BIOCHEMISTRY, vol. 45, p. 11879-11884, ISSN: 0006-2960
  • PERUGINO G, FALCICCHIO P, CORSARO MM, MATSUI I, PARRILLI M, ROSSI M, and MORACCI M (2006). Preparation of a glycosynthase from the b-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 24, p. 23-29, ISSN: 1024-2422
  • COBUCCI-PONZANO B, PERUGINO G, DI LAURO B, MAZZONE M, ROSSI M, and MORACCI M (2005). Glycoside hydrolases from hyperthermophilic Archaea. In: Biocatalysis: Chemistry and Biology. p. 127-149, Eds Research Signpost
  • FALABELLA P, PERUGINO G, CACCIALUPI P, RIVIELLO L, VARRICCHIO P, TRANFAGLIA A, ROSSI M, MALVA C, GRAZIANI F, MORACCI M, and PENNACCHIO F (2005). A novel Fatty Acid Binding Protein produced by teratocytes of the aphid parasitoid Aphidius ervi. INSECT MOLECULAR BIOLOGY, vol. 14, p. 195-205, ISSN: 0962-1075
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, GIORDANO A, TRINCONE A, and ROSSI M (2005). Recent developments in the synthesis of oligosaccharides by hyperthermophilic glycosidases. In: Handbook of Carbohydrate Engineering. Eds Taylor and Francis Group
  • PERUGINO G, COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2005). Recent advances in the oligosaccaride synthesis promoted by catalytically engineered glycosidases. ADVANCED SYNTHESIS & CATALYSIS, vol. 347, p. 941-950, ISSN: 1615-4150
  • TRINCONE A, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2005). Highly productive autocondensation and transglycosylation reactions with Sulfolobus solfataricus glycosynthase. CHEMBIOCHEM, vol. 6, p. 1431-1437, ISSN: 1439-4227
  • GLOSTER TM, ROBERTS S, DUCROS V.M, PERUGINO G, ROSSI M, HOOS R, MORACCI M, VASELLA A, and DAVIES GJ (2004). Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors. BIOCHEMISTRY, vol. 43, p. 6101-6109, ISSN: 0006-2960
  • PERUGINO G, TRINCONE A, ROSSI M, and MORACCI M (2004). Oligosaccharide synthesis by glycosynthases. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 31-37, ISSN: 0167-7799
  • COBUCCI-PONZANO B, PERUGINO G, TRINCONE A, MAZZONE M, DI LAURO B, GIORDANO A, ROSSI M, MORACCI M (2003). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1029-2446, doi: 10.1080/10242420310001618555
  • Cobucci Ponzano, Beatrice, PERUGINO, GIUSEPPE, Trincone, Antonio, Mazzone, Marialuisa, DI LAURO, BARBARA, Giordano, Assunta, ROSSI, MOSE’, MORACCI, Marco (2003). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1024-2422, doi: 10.1080/10242420310001618555
  • PERUGINO G, TRINCONE A, GIORDANO A, VAN DER OOST J, KAPER T, ROSSI M, and MORACCI M (2003). The activity of hypethermophilic glycosynthases is significantly enhanced at acidic pH. BIOCHEMISTRY, vol. 42, p. 8484-8493, ISSN: 0006-2960
  • TRINCONE A, GIORDANO A, PERUGINO G, COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2003). Glycosyl hydrolases from Sulfolobus solfataricus: enzymology and applications. In: Recent Research Developments in Biotechnology & Bioengineering. p. 129-145, Eds Research Signpost
  • TRINCONE A, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2003). Glycosynthase-catalysed syntheses at pH below neutrality. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 13, p. 4039-4042, ISSN: 0960-894X
  • TRINCONE A, PAGNOTTA E, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2003). Enzymatic synthesis of 2-deoxyglycosides using the beta-glycosidase of the archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 17-24, ISSN: 1024-2422
  • MORACCI M, TRINCONE A, COBUCCI-PONZANO B, PERUGINO G, CIARAMELLA M, and ROSSI M (2001). Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases from Sulfolobus solfataricus. EXTREMOPHILES, vol. 5, p. 145-152, ISSN: 1431-0651
  • POUWELS J, MORACCI M, COBUCCI-PONZANO B, PERUGINO G, VAN DER OOST J, KAPER T, LEBBINK J.H, DE VOS W.M, CIARAMELLA M, and ROSSI M (2000). Activity and stability of hyperthermophilic enzymes: a comparative study on two archaeal beta-glycosidases. EXTREMOPHILES, vol. 3, p. 157-164, ISSN: 1431-0651
  • TRINCONE A, PERUGINO G, ROSSI M, and MORACCI M (2000). A novel thermophilic glycosynthase that effects branching glycosylation. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 10, p. 365-368, ISSN: 0960-894X
  • MORACCI M, TRINCONE A, PERUGINO G, CIARAMELLA M, and ROSSI M (1998). Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles. BIOCHEMISTRY, vol. 37, p. 17262-17270, ISSN: 0006-2960
 

Selected Publications
(full list available at CNR People)

Page Contents

Related Contents

Other Personal Pages