Lisa Tuppo
Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132481
E-mail: lisa.tuppo@ibbr.cnr.it
Address: Institute of Biosciences and BioResources, Division of Naples, National Research Council,
via Pietro Castellino, 111 - 80131 Napoli, Italy
Tel: +39 081 6132712
E-mail: lisa.tuppo@ibbr.cnr.it
Education and Training
2004 Degree in Biological Science at the University "Federico II" of Naples with 110/110 cum laude.
2006 Awarded a fellowship of the Centro di Competenza “Bioteknet” of Campania Region, at theItalian National Research Council (CNR), Institute of Protein Biochemistry (IBP), Naples, Italy.
2007-2010 Project-linked contracts with the IDI-IRCCS, Rome, Italy, in the framework of the research programme at CNR, Institute of Protein Biochemistry, Naples, Italy.
2010-2013 Researcher of the IDI-IRCCS, Rome, Italy, in the framework of the research programme at CNR, Institute of Protein Biochemistry, Naples, Italy.
2014 PhD in Cell Biochemistry, (26th cycle) at the Second University of Naples (SUN).
2015-2017 Professional collaboration with Associated Centers of Molecular Allergology (CAAM, Latina, Italy) as an expert in protein biochemistry at CNR, Institute of Biosciences and BioResources, Naples.
2017-2020 Permanent contract at Allergy Data Laboratories (ADL S.r.l., Latina, Italy) as expert in protein biochemistry for a scientific collaboration project with the Institute of Biosciences and BioResources, CNR, Naples.
2020-to date Permanent position as Researcher at the Italian National Research Council (CNR), Institute of Biosciences and BioResources, UOS Naples.
Research Interests
Our research interests are focused on the structural, functional and immunological characterisation of proteins and peptides from food and environmental sources, with potential effects on human health. In the last few years, our studies have involved the field of molecular allergology with the isolation of new and already known allergenic proteins. These molecules are purified mostly from the natural source by classical biochemical methods and identified by direct amino acid sequencing. Their characterisation includes the study of the primary and secondary structures (also post-translational modifications) as well as the investigation of their stability in different environmental/experimental conditions, including heat stability and gastrointestinal digestion. The biological function involves kinetic studies of enzymatic activities.
The research activity carried out has led to the acquisition, over the years, of specific knowledge in the development of specific protocols for preparation of protein allergenic solutions (extract and purified molecules) suitable for commercial diagnostic systems or for research use. Analysis of the protein and allergen profile in different foods using immunochemical methods. Moreover, study of the influence of several factors (ripening stage, post-harvest treatments, cultivar, etc.) on the variation of the protein and allergen profiles in plant foods.
Current main collaborations
Prof. Adriano Mari, Associated Centers for Molecular Allergology (CAAM), Rome (www.caam-allergy.com/)
Prof. Maksymilian Chruszcz, Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, USA (www.chem.sc.edu/)
Dr Silvia Pasquariello, Domenico De Lucia S. p. A., San Felice a Cancello, Caserta, Italy
(http://www.domenicodelucia.it/en/)
Publications
Please find the full list of my publications at the following URL:
https://scholar.google.com/citations?user=MfNi0EAAAAAJ&hl=en
