Paolo Iovieno

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Education

April 2013. Ph.D in Agrochemistry and Agrobiology for the Genetic Improvement of Plants and Horticulture, University of Naples Federico II. Dissertation title: “Integrated approaches to the study of physiological and molecular mechanisms of response to water and thermal stress in tomato”.

June 2008. Master in Plant Genomics, Institute of Plant Genetics, Italian National Council of Research (CNR-IGV). Main subject: high throughput genomics for plant improvement and certification.

June 2006. MSc in Biology, University of Naples Federico II. Experimental dissertation in biochemical methodologies. Dissertation title: “Characterization of molecular interaction between the transcriptional factor LRS14 and BALD16 of the archaeon Sulfolobus Solfataricus”. Final Voting 110/110 cum laude.

Research Experiences

July 2020. Researcher at CNR-IBBR, Research Division of Sesto Fiorentino.

Dicember 2019 - June 2020. Researcher at ENEA, Italian National Agency for New Technologies Energy and Sustainable Economic Development, Trisaia Research Centre (Italy). Research division:of Bioenergy, Bio-refinery and Green Chemistry (DTE-BBC). Research interest in production of bioenergy, bioproducts and biocatalysts, environmental risk assessment of RNA interfering applications.

October 2016 - December 2017. Postdoctoral researcher, Department of Plant, Soil and Food Science, University of Bari “Aldo Moro”. Investigation on characterization of MLO powdery mildew susceptibility in Cucurbitaceae by means of heterologous overexpression and molecular study of Apulian horticultural germplasm.

March - April 2016. Guest Researcher, Department of Plant and Environmental Sciences, University of Copenhagen, within the framework of the Cost Action FA1208-id n° 010316-071368. Research interest in identification of zucchini MLO powdery mildew susceptibility factors and set-up of genome editing protocols for C. pepo.

October 2013 - November 2015. Postdoctoral researcher, Department of Agricultural Sciences of the University of Naples Federico II. Investigation on identification of genes of resistance and Research interests in susceptibility to ZYMV and powdery mildew in zucchini. Study of zucchini genome. Editing of plant genome using Crispr/Cas9 technology.

March 2010 - April 2013. PhD student, University of Naples Federico II. Investigation on physiological, biochemical and molecular response to abiotic stresses (drought and high temperatures) in Solanum Lycopersicum.

September 2008 - April 2013. Research fellow, CNR-Institute of Plant Genetics (IGV), Portici. Research about molecular mechanisms of response to abiotic stresses (drought and high temperastures) in Solanum Lycopersicum.

January - June 2008. Research Intern, CNR-Institute of Plant Genetics (IGV), Portici. Functional analysis of some tomato genes involved into thermo-tolerance using a gain-of-function approach throughput stable transformation mediated by Agrobacterium tumefaciens.

March 2005 - June 2006. Undergraduate research assistant, University of Naples Federico II.
Study on molecular mechanism of detoxification from aromatic aldehydes in Sulfolobus solfataricus using biotechnological tools.

Main research topics

My research focuses on: the analysis of molecular markers for characterization of plant DNA diversity; the identification and the functional analysis of genes involved in powdery mildew in Cucurbitaceae; the molecular mechanisms of response to abiotic stresses (drought and high temperastures) in tomato; the genome editing using the CRISPR/Cas9 technology.

Publications

• Assessment of Genetic Diversity of the "Acquaviva Red Onion" (Allium cepa L.) Apulian Landrace (2020). Ricciardi L., Mazzeo R., Marcotrigiano A.R, Rainaldi G., Iovieno P., Zonno V., Pavan S, Lotti C. Plants (Basel) 9 (2), 260. DOI:10.3390/plants9020260.
• Evolutionary conservation of mlo gene promoter signatures (2019). Andolfo G., Iovieno P., Ricciardi L., Lotti C., Filippone E., Pavan S, Ercolano M. R BMC Plant Biology 19 (1), 150. DOI: 12870-019-1749-3.
• Physiological, biochemical and molecular responses to water stress and re-hydration in mediterranean adapted tomato landraces (2018). Giorio, P., Gianpiero, G. Mistretta C., Sellami, M., Oliva M., Punzo P., Iovieno P., Arena C., De Maio A., Grillo S., Albrizio R. Plant Biology 20 (6), 995-1004. DOI: 10.1111/plb.12891.
• Response mechanisms induced by exposure to high temperature in anthers from thermo-tolerant and thermo-sensitive tomato plants: a proteomic perspective (2018). Mazzeo M. F., Cacace G., Iovieno P., Massarelli I., Grillo S., Siciliano R. A. Plos ONE. 13 (7), e0201027. DOI: 10.1371/journal.pone.0201027.
• Genetic, bio-agronomic, and nutritional characterization of kale (Brassica oleracea l. var. Acephala) diversity in Apulia, southern Italy (2018). Lotti C., Iovieno P., Centomani I., Marcotrigiano A.R., Fanelli V., Mimiola G., Summo C., Pavan S., Ricciardi L. Diversity, 10 (2), 25. DOI: 10.3390/d10020025.
• Inheritance analysis and identification of snp markers associated to ZYMV resistance in Cucurbita pepo (2017). Capuozzo C.*, Formisano G.*, Iovieno P., Andolfo G., Tomassoli L., Barbella M.M, Pico B., Paris H.S., Ercolano M.R. Molecular breeding. 37 (8), 99. DOI: 10.1007/s11032-017-0698-5.
• Genome-editing technologies for enhancing plant disease resistance (2016). Andolfo G.*, Iovieno P.*, Frusciante L., Ercolano M.R. Frontiers in plant science. 7, 1813. DOI: 10.3389/fpls.2016.01813.
• Identification and functional inference on Viridiplantae Mlo-family (2016). Iovieno P., Bracuto V., Pavan. S., Lotti C., Ricciardi L., Andolfo G. Journal of plant pathology. 98, 587-594. DOI: 10.4454/JPP.V98I3.027.
• Transcriptomic changes drive physiological responses to progressive drought stress and rehydration in tomato (2016). Iovieno P., Punzo P., Guida G., Mistretta C., Van Oosten M. J., Nurcato R., Bostan H., Colantuono C., Costa A., Bagnaresi P., Chiusano M.L., Albrizio R., Giorio P., Batelli G., Grillo S. Frontiers in plant science. 7, 371. DOI: 10.3389/fpls.2016.00371.
• Structure, evolution and functional inference on of the MLO gene family in three cultivated Cucurbitaceae spp. (2015). Iovieno P.*, Andolfo G.*, Schiavulli A., Catalano D, Ricciardi L, Frusciante L., Ercolano M.R, Pavan S. BMC Genomics. 16 (1), 1112. DOI:10.1186/s12864.
• Production of tomato transgenic plants for the functional analysis of the transcription factor HSFA2 gene in response to heat stress (2010). Iovieno P., Nurcato R., Massarelli I., Leone A., Grillo S. Minerva Biotecnolgica; 22 Suppl. 1 No. 2:38-9.

*The authors contribuited to the work equally.

Patent

Genes of Susceptibility to Powdery Mildew. Mildew (2018). Iovieno P., Andolfo G., Ercolano M. R., Frusciante L. University of Naples Federico II. Patent n. 102015000063332. Country: Italy

https://scholar.google.com/citations?hl=en&user=AufWNUAAAAAJ

Selected Publications

(full list available at CNR People)

Biofortification of tomato using genome editing

Iovieno P, Capuana M, Scarano A, Santino A, Basso MF, Martinelli F

Year: 2023