Roberto De Michele
Role: Senior Researcher
Section: Researchers and Technologists
Division: Palermo
Tel: (39) 091-6574578
E-mail: roberto.demichele@ibbr.cnr.it
Research Interest
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Development and use of genetically encoded fluorescent sensors. In particular, transporter activity sensors for ammonium (AmTracs) for studying ammonium transport in plants and during symbiosis.
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Role of reactive oxygen and nitrogen species (ROS and RNS) in plant responses to biotic and abiotic stresses and development.
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Plant responses to heavy metal and programmed cell death (PCD)
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Molecular aspects of senescence
Education
2005 PhD in Biotechnology, Department of Biology, University of Padova (Italy).
2004 Visiting exchange student at University of California, Berkeley (U.S.)
2001 Master Degree (Laurea) in Biological Science, University of Palermo, Italy.
1999 Visiting exchange (Erasmus) student at University of Wales Swansea (U.K.)
Professional experiences
2020- Visiting scientist, Cambridge University, Regno Unito
2019- Mercator Fellow visiting scientist Heinrich Heine University, Dusseldorf, Germania
2018- Visiting scientist, Cambridge University, Regno Unito
2010- Researcher at CNR, Institute of Biosciences and Bioresources, Palermo, Italy
2010- 2012 Postdoctoral fellow in the laboratory of Wolf Frommer, Department of Plant Biology, Carnegie Institution for Science, Stanford, USA.
2009- 2010 Research fellow at CNR, Institute of Plant Genetics of Palermo, Italy.
2008- 2009 Postdoctoral fellow in the laboratory of M.Carmen Martinez, Department of Biochemistry and Molecular Biology, Universitat Autonoma de Barcelona, Spain.
2007 Research fellow in the laboratory of Prof. Michela Zottini, Department of Biology, University of Padova, Italy.
2005-2007 Postdoctoral fellow in the laboratory of Fiorella Lo Schiavo, Department of Biology, University of Padova, Italy.
2005 Research fellow in the laboratory of Prof. Mario Terzi, Department of Biology, University of Padova, Italy.
2004 Visiting student in the laboratory of Prof. Russell Jones, Department of Plant and Microbial Biology, University of California, Berkeley (U.S.A.).
2001 Research fellow at CNR (Italian National Research Council) , Institute of Plant Genetics of Palermo, Italy.
Selected Publications
(full list available at CNR People)
Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives
Sadoine M, De Michele R, Åàupunski M, Grossmann G, Castro-Rodríguez V
Year: 2023
Different cell Types Affect the Transition from Juvenile to Mature Phase in Citrus Plants Regenerated through Somatic Embryogenesis
Catalano C, Abbate L, Fatta Del Bosco S, Motisi A, Carimi F, De Michele R, Mercati F, D’onghia AM, Carra A
Year: 2022
Cell death induced by mycotoxin fumonisin B1 is accompanied by oxidative stress and transcriptional modulation in Arabidopsis cell culture
Lanubile A, De Michele R, Loi M, Fakhari S, Marocco A, Paciolla C
Year: 2022
Multiple fungal diseases resistance induction in Cucumis melo through co-transformation of different pathogenesis related (PR) protein genes
Raji MR, Lotfi M, Tohidfar M, Ramshini H, Sahebani N, Aalifar M, Baratian M, Mercati F, De Michele R, Carimi F
Year: 2022
Phylogenetic relationship among wild and cultivated grapevine in Sicily: a hotspot in the middle of the Mediterranean Basin
De Michele R, La Bella F, Gristina AS, Fontana I, Pacifico D, Garfi G, Motisi A, Crucitti D, Abbate L, Carimi F
Year: 2019
Hydrogen sulfide directs metabolic flux towards the lignan biosynthesis in Linum album hairy roots
Fakhari S, Sharifi M, De Michele R, Ghanati F, Safaie N, Sadeghnezhad E
Year: 2019
Transcriptome analysis and codominant markers development in caper, a drought tolerant orphan crop with medicinal value
Mercati F, Fontana I, Gristina AS, Martorana A, El Nagar M, De Michele R, Fici S, Carimi F
Year: 2019
Transcriptomic analysis reveals the roles of detoxification systems in response to mercury in Chromera velia
Sharaf A, De Michele R, Sharma A, Fakhari S, Oborník M
Year: 2019
Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
Ast C, Foret J, Oltrogge LM, De Michele R, Kleist TJ, Ho CH, Frommer WB
Year: 2017
Urgent need for preservation of grapevine (Vitis vinifera L. subsp. vinifera) germplasm from small circum-Sicilian islands as revealed by SSR markers and traditional use investigations
Gristina AS, De Michele R, Garfì G, La Mantia T, Fontana I, Spinelli P, Motisi A, Carimi F
Year: 2017
Free-flow electrophoresis of plasma membrane vesicles enriched by two-phase partitioning enhances the quality of the proteome from Arabidopsis seedlings
De Michele R, Mcfarlane HE, Parsons HT, Meents MJ, Lao J, González Fernández-Niño SM, Petzold CJ, Frommer WB, Samuels AL, Heazlewood JL
Year: 2016
Single-fluorophore membrane transport activity sensors with dual-emission read-out
Ast C, De Michele R, Kumke MU, Frommer WB
Year: 2015
Mitochondrial biosensors
De Michele R, Carimi F, Frommer WB
Year: 2014
Exploring the plant endomembrane by free-flow electrophoresis
González Fernández-Niño S, Parsons H, De Michele R, Heazlewood J
Year: 2014
Linking protein kinase CK2 and auxin transport
Marquès-Bueno MM, Moreno-Romero J, Abas L, de Michele R, Martínez MC
Year: 2014
Fluorescent sensors reporting the activity of ammonium transceptors in live cells
De Michele R, Ast C, Loqué D, Ho C-H, Andrade SLA, Lanquar V, Grossmann G, Gehne S, Kumke MU, Frommer WB
Year: 2013
Habitat features and genetic integrity of wild grapevine Vitis vinifera L. subsp. sylvestris (C.C. Gmel.) Hegi populations: A case study from Sicily
Garfì G, Mercati F, Fontana I, Collesano G, Pasta S, Vendramin GG, De Michele R, Carimi F
Year: 2013
Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization
Wang Q, Zhao Y, Luo W, Li R, He Q, Fang X, De Michele R, Ast C, von Wiren N, Lin J
Year: 2013
Ammonium and urea transporter inventory of the Selaginella and Physcomitrella Genomes
De Michele R, Loqué D, Lalonde S, Frommer WB
Year: 2012
S-Nitrosoglutathione is a component of wound- and salicylic acid-induced systemic responses in Arabidopsis thaliana
Espunya MC, De Michele R, Gómez-Cadenas A, Martínez MC
Year: 2012
Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’ as revealed by microsatellite markers
Carimi F, Mercati F, De Michele R, Fiore MC, Riccardi P, Sunseri F
Year: 2011
A dominant negative mutant of protein kinase CK2 exhibits altered auxin responses in Arabidopsis
Marquès-Bueno MM, Moreno-Romero J, Abas L, De Michele R, Martínez MC
Year: 2011
Role of nitric oxide in programmed cell death
Zottini M, Costa A, De Michele R, Lo Schiavo F
Year: 2010
Cross Talk between Nitric Oxide and Growth Regulators. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues
De Michele R, Zottini M, Lo Schiavo F, Carimi F
Year: 2006
