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Description
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The specific objectives of the research proposal are: 1) The genetic characterization with already available molecular markers (nSSRs and cpSSRs) of at least 6 populations (3 from the Apennines and 3 from Montenegro, 50 geo-referenced individuals per populations) of Aa, Pa, Pn and Ph; 2) The genetic characterization with nSSRs and cpSSRs of at least 3 populations (2 continuous and 1 peripheral and fragmented, 50 geo-referenced individuals per populations) of Pp, with test for transferability of markers from other Pinaceae; at least 12 markers, combining nSSRs and cpSSRs will be tested; 3) After having added the newly genotyped Aa, Pa, Pn and Ph populations to already existing datasets, estimating the existing spatial and non-spatial genetic structure at the biogeographical level; 4) Estimating divergence time through ABC-based approaches (e.g. DIYABC or popABC software, depending on the level of differentiation found); 5) Estimating migration rates through Bayesian approaches (e.g. Migrate software); 6) Comparing parameters describing genetic variation, divergence and inbreeding between continuous and fragmented Pp populations; 7) Propose actions for the conservation of the genetic resources of the selected species in Italy and in Montenegro; 8) Strengthen the scientific collaboration between the two countries and favour the exchange of scientists and students |
