Genomic Lab - Florence

The ION Torrent PGM2 multifunction station at the IBBR/UOS Florence

Description

The Genomic laboratory at IBBR in Florence is equipped with the most advanced facilities to perform DNA and RNA sequencing, genotyping and gene expression.

It is constituted by Ion Torrent™ PGM, Ion OneTouch2, Ion OneTouch ES, Ion Chef System, and Applied Biosystems 3500 capillary sequencer.

The Ion PGM™ System makes affordable, high-quality next-generation sequencing and can perform a variety of targeted gene sequencing applications.

Next-generation sequencing (NGS) can provide a global genetic survey of the genome and transcriptome, or it can be restricted to specific regions of the genome or transcriptome. Identify specific changes in DNA by rapidly and simultaneously sequencing multiple gene targets within multiple samples (Target hundreds of genes simultaneously with ultrahigh-multiplex PCR using up to 6.144 primer pairs in a single primer pool). RNA sequencing using NGS technology not only provides a digital representation of absolute expression, but also can identify and characterize low-abundance transcripts.

These facilities permit to make the investigation starting from only 10 ng of DNA or 5 ng of RNA.

Reference Person

Dr. Cristina Vettori (IBBR/UOS Florence - see personal page).