DraLF-PolI: a good candidate for a new cutting-edge Room-Temperature LAMP

Mariarita De Felice 
IBBR - UOS Napoli - Italy

November 06, 2024 (11:30-12:30)
Webinar Link: https://teams.microsoft.com/l/meetup-join/19:meeting_ZWVmOGM2NjAtODczNi00MTg3LThjMmMtOGE1YTRjY2UwYTM3@thread.v2/0?context={"Tid":"34c64e9f-d27f-4edd-a1f0-1397f0c84f94","Oid":"c9f800b1-fb5c-40ef-8448-c7bbb3f5a393"}

Abstract: In recent years, the Loop-mediated isothermal amplification (LAMP) technique, designed for microbial pathogen detection, have acquired fundamental importance in the biomedical field, providing rapid and precise responses. However, it still has some drawbacks, including the main one due to the need for a thermostatic block, necessary to reach 63 °C, which is BstI DNA polymerase working temperature. Here we report the identification and characterization of the DNA polymerase I Large Fragment from Deinococcus radiodurans (DraLF-PolI) that functions at room temperature and is resistant to various environmental stress conditions. We demonstrated that DraLF-PolI displays efficient catalytic activity over a wide range of temperature and pH, maintains its activity even after storage under various stress conditions, including desiccation, and retains its strand displacement activity required for isothermal amplification technology. All these characteristics make DraLF-PolI an excellent candidate for a cutting egde room temperature LAMP that promises to be very useful for the rapid and simple detection of pathogens at the Point of Care

Author's Info: https://ibbr.cnr.it/ibbr/info/people/mariarita-de-felice